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Creation and Maintenance of a Living Biobank – How We Do It

JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro

Creation and Maintenance of a Living Biobank – How We Do It

Creation and Maintenance of a Living Biobank – How We Do It

DOI:

10.3791/62065-v

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13:08 min

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April 10, 2021

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Florian Bürtin, Stephanie Matschos, Friedrich Prall, Christina S. Mullins, Mathias Krohn, Michael Linnebacher

¹Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, University of Rostock, ²Molecular Oncology and Immunotherapy, Department of General, Visceral, Vascular and Transplantation Surgery,University Medical Center Rostock, ³Institute of Pathology,University Medical Center Rostock

Capitoli

00:00Introduction
00:36Sample Acquisition
02:41Tissue Processing
05:43Cell Culture
07:40Generation of Patient-Derived Xenografts
11:16Explantation of PDX
11:44Representative Results
12:41Conclusion

Summary

Traduzione automatica

In the following work, we describe the consecutive steps necessary for the establishment of a large biobank of colorectal and pancreatic cancer.

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Keywords: Living Biobank Patient-derived Models Precision Medicine Sample Acquisition Serum Processing PBL Isolation Snap Freezing

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