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भ्रूण Microinjection और नॉकआउट उत्परिवर्ती CRISPR / Cas9 जीनोम-संपादित हेलिकोवेर्पा Armigera (Hübner) की पहचान
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1School of Chemical and Environmental Engineering,China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences
Capitoli
- 00:05Introduction
- 00:29Design of Gene-Specific Primers and Preparation of sgRNA and Embryos
- 02:18Microinjection of Embryos
- 03:47Post-Injection Insect Rearing
- 04:41Knock-Out Mutation Detection
- 05:27Results: Selection and Synthesis of Target sgRNAs and Detection of Mutants by PCR and Gel Electrophoresis
- 06:08Conclusion
