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Discovery of Metastatic Regulators using a Rapid and Quantitative Intravital Chick Chorioallantoic Membrane Model
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Capitoli
- 00:05Introduction
- 00:41Intravenous Injection of Cancer Cells for Metastatic Colony Formation
- 03:06Embryo Maintenance During the Metastatic Colony Growth and their Isolation
- 05:26Results: Metastatic Colony Isolation for Different Steps of the Screening Protocol
- 06:34Conclusion
This is an effective method to screen for suppressors or drivers of cancer metastasis. Cells, transduced with an expression library, are injected into the chicken chorioallantoic membrane vasculature to form metastatic colonies. Colonies having decreased or increased invasiveness are excised, expanded, reinjected to confirm their phenotype, and finally, analyzed using high throughput sequencing.
