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Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
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Capitoli
- 00:04Introduction
- 01:04Sample Preparation for Live Imaging
- 01:57Sample Preparation for Immunofluorescence Microscopy
- 04:20Repositioning of Embryo to an Anatomically Standard Position Using Fiji/ImageJ
- 07:19Sample Preparation for Optical Projection Tomography
- 10:29Results: Visualization and Analysis of Three and Four-Dimensional Image Data of Mouse Embryos
- 12:23Conclusion
Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.
