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在合并臭氧和LPS诱导穆林急性肺损伤期间可视化肺细胞适应
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
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在合并臭氧和LPS诱导穆林急性肺损伤期间可视化肺细胞适应

DOI:
10.3791/62097-v

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14:48 min

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March 21, 2021

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, ,
1Small Animal Clinical Sciences, Western College of Veterinary Medicine,University of Saskatchewan

Capitoli

  • 00:00Introduction
  • 01:33Protocol: Chapter I. Ozone and LPS Exposures for Induction of Murine ALI
  • 02:33Protocol: Chapter II. Sample Collection
  • 08:55Protocol: Chapter III. Sample Processing
  • 10:17Representative Results
  • 14:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

臭氧和细菌内毒素暴露的小鼠的结合显示广泛传播的细胞死亡，包括嗜中性粒细胞死亡。我们观察到细胞适应，如细胞骨质跛脚膜的中断，复杂V ATP合成酶亚单位β和血管他汀在支气管-阿尔韦奥拉厕所的细胞表达增加，抑制肺免疫反应和延迟中性粒细胞招募。

Tags

Lung InflammationAcute Lung InjuryOzone ExposureLPS InstillationBronchoalveolar Lavage Fluid (BAL)Lung Vascular Perfusate (LVP)Lung CryosectionsPeripheral BloodBone MarrowMouse ModelImagingFluorescence Microscopy

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