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En proinflammatorisk, degenerativ organkulturmodel til at simulere tidlig intervertebrale diskose.
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Medicina
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JoVE Journal Medicina
A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

En proinflammatorisk, degenerativ organkulturmodel til at simulere tidlig intervertebrale diskose.

DOI:
10.3791/62100-v

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05:46 min

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February 14, 2021

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, , , , , , ,
1AO Research Institute Davos, Davos, Switzerland, 2Department of Orthopedics and Trauma Surgery, Medical Centre-Albert-Ludwigs-University of Freiburg, Faculty of Medicine,Albert-Ludwigs-University of Freiburg, Freiburg, Germany

Capitoli

  • 00:04Introduction
  • 00:38Dissection of the Bovine Intervertebral Disc
  • 02:27IVD Culture and Loading
  • 03:36Intradiscal TNF-α Injection
  • 04:13Results: Assessment of Intervertebral Disc Degeneration Caused by Degenerative Loading, Limited Nutrition, and TNF-α Injection
  • 04:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol præsenterer en ny eksperimentel model af proinflammatorisk, degenerativ kvægorgankultur for at simulere tidlig intervertebral diskdegeneration.

Tags

Intervertebral Disc DegenerationOrgan Culture ModelBioreactorDisc CultureDisc LoadingDegenerative Disc DiseaseIn Vitro ModelBovine TailDisc HeightDisc DiameterDisc Microenvironment

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