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The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila
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JoVE Journal Neuroscienze
The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila

DOI:
10.3791/62105-v

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06:06 min

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December 14, 2020

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, , , ,
1Department of Neuroscience,Washington University School of Medicine

Capitoli

  • 00:04Introduction
  • 00:51Recording Sleep
  • 02:15Sleep Deprivation and Recovery
  • 03:41Results: Sleep Deprivation Using the Sleep Nullifying APparatus (SNAP)
  • 05:18Conclusion

Summary

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Sleep deprivation is a powerful tool to investigate sleep function and regulation. We describe a protocol to sleep deprive Drosophila using the Sleep Nullifying Apparatus, and to determine the extent of rebound sleep induced by deprivation.

Tags

Keywords: Sleep DeprivationSleep RestrictionDrosophilaSleep HomeostasisSleep RegulationSNAP (Sleep Nullifying Apparatus)Activity MonitoringLocomotor ActivitySleep BoutSleep Recovery

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