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Immunology and Infection

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JoVE Journal Immunology and Infection
Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection
 

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection

Article DOI: 10.3791/62115-v • 07:36 min • February 28th, 2021
February 28th, 2021 •
*1, *1, 1, 2, 3, 1,4, 1,4, 5, 2,6, 1,2,6, 1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
* These authors contributed equally

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This protocol details an investigation of the early interactions between virally infected nasal epithelial cells and innate cell activation. Individual subsets of immune cells can be distinguished based on their activation in response to viral infections. They can then be further investigated to determine their effects on early antiviral responses.

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Contact-Free Co-Culture Model Innate Immune Cell Activation Respiratory Virus Infection Epithelial Cells Innate T Cells Human Sources Anti-influenza Therapy Antiviral Therapy Preventive Measures Nasal Epithelial Cells Trypsin EDTA Virus Stock
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