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Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.
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JoVE Journal Medicina
Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

DOI:
10.3791/62129-v

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08:37 min

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March 03, 2021

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, , , , , , ,
1Division of Regenerative Medicine, Center for Molecular Medicine,Jichi Medical University, 2Department of Pediatrics,Jichi Medical University, 3Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 4Department of Precision Mechanics, Faculty of Science and Engineering,Chuo University

Capitoli

  • 00:04Introduction
  • 01:04Human Pluripotent Stem Cell Differentiation
  • 03:15Patterned Pluripotent Stem Cell-Derived Cardiomyocyte (PSC-CM) Culture
  • 05:07Time-Lapse Sarcomere Imaging
  • 05:58Time-Lapse Imaging Analysis
  • 06:36Results: Representative Sarcomere Shortening Evaluation
  • 07:56Conclusion

Summary

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This method can be used to examine sarcomere shortening using pluripotent stem cell-derived cardiomyocytes with fluorescent-tagged sarcomere proteins.

Tags

Keywords: Sarcomere ShorteningPluripotent Stem Cell-derived CardiomyocytesFluorescent-tagged Sarcomere ProteinsAAV-based TransductionInduced Pluripotent Stem CellsCardiomyopathy TherapySkeletal Muscle DysfunctionMyopathyLaminin-511 E8ROCK InhibitorGSK-3 InhibitorPorcupine Inhibitor

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