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使用GAL4-UAS系统在冈比亚按蚊的功能遗传学中
JoVE Journal
Genetica
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JoVE Journal Genetica
Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae
DOI:

06:29 min

April 15, 2021

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Capitoli

  • 00:05Introduction
  • 00:49Collection of Pupae for Microscopic Characterization and Identification of Fluorescent Markers
  • 01:45Sexing Pupae
  • 02:25Setting Up GAL4-UAS (Upstream Activation Sequences) System Crosses and Inducing Egg Laying
  • 03:20Embryo Fixation and Bleaching
  • 04:15Results: Analysis of Anopheles gambiae Pupae Expressing Fluorescent Markers Driven by 3xP3 Promoter, Tissue-specific Expression Visualization, and Embryo Clearing
  • 05:53Conclusion

Summary

Traduzione automatica

二分GAL4-UAS系统是一种多功能工具,用于以受控的时空方式修饰基因表达,从而允许在 冈比亚按蚊中进行功能性遗传分析。使用该系统所描述的程序是半标准化的克隆策略,性别化和蛹荧光蛋白标志物的筛选以及胚胎固定。

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