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Assessing Biofilm Dispersal in Murine Wounds
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Assessing Biofilm Dispersal in Murine Wounds
DOI:

12:18 min

August 07, 2021

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Capitoli

  • 00:00Introduction
  • 01:24Dorsal Full Thickness Excision Surgery
  • 03:25In vivo Treatment and Assessment of Dispersal
  • 07:04Ex vivo Assessment of Dispersal
  • 09:12Results: Enzymatic Dispersal Leads to Bacterial Dissemination in the Host
  • 10:55Conclusion

Summary

Traduzione automatica

Here, we describe ex vivo and in vivo methods for assessing bacterial dispersal from a wound infection in mice. This protocol can be utilized to test the efficacy of topical antimicrobial and anti-biofilm therapies, or to assess the dispersal capacity of different bacterial strains or species.

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