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具有可移动XY阶段的平台孵化器：实现蛋白质细胞内快速光化学氧化的新平台

JoVE Journal
Biochimica

This content is Free Access.

JoVE Journal Biochimica

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

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具有可移动XY阶段的平台孵化器：实现蛋白质细胞内快速光化学氧化的新平台

DOI:

10.3791/62153-v

•

05:50 min

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May 17, 2021

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Danté Johnson, Benjamin Punshon-Smith, Jessica A. Espino, Anne Gershenson, Lisa M. Jones

¹Department of Pharmaceutical Sciences,University of Maryland Baltimore, ²Technology Research Center,University of Maryland Baltimore County, ³Department of Biochemistry and Molecular Biology,University of Massachusetts

Capitoli

00:04Introduction
00:45Setting Up the Platform Incubator for IC-FPOP
02:08IC-FPOP in the Platform Incubator
03:41Results: Analysis of Cell Transfection Efficiency, Proteins Modified in Single Cell Flow-System and PIXY, and Localization of IC-FPOP Modifications
05:22Conclusion

Summary

Traduzione automatica

一种新的静态平台用于利用一种称为蛋白质细胞内快速光化学氧化（IC-FPOP）的蛋白质足迹技术，对原生细胞环境中的蛋白质结构和相互作用部位进行特征。

Tags

Keywords: Platform Incubator Movable XY Stage In-cell FPOP Protein-ligand Interactions Protein-protein Interactions Conformational Changes Automated Six-well Plate Cell Culture Hydrogen Peroxide Quench Buffer Peristaltic Pumps Laser Cell Scraper Centrifugation

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