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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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Biologia
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JoVE Journal Biologia
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

DOI:
10.3791/62175-v

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10:59 min

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August 21, 2021

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, , ,
1Institute of Pathology, Faculty of Medicine,University of Szeged

Capitoli

  • 00:05Introduction
  • 01:44Immunodetection of Specific Proteins
  • 05:08Chromatin Immunoprecipitation
  • 09:06Representative Results
  • 10:28Conclusion

Summary

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This article introduces essential steps of immunostaining and chromatin immunoprecipitation. These protocols are commonly used to study DNA damage-related cellular processes and to visualize and quantify the recruitment of proteins implicated in DNA repair.

Tags

Keywords: DNA DamageImmunostainingChromatin ImmunoprecipitationDNA RepairLaser MicroirradiationEndonucleaseDouble-strand BreaksNeocarzinostatinU2OS CellsCell CultureFixation

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