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Immunostaining af helmonteringskinner med KLARHED Vævsclearingmetoden
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Neuroscienze
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JoVE Journal Neuroscienze
Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

Immunostaining af helmonteringskinner med KLARHED Vævsclearingmetoden

DOI:
10.3791/62178-v

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09:01 min

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March 06, 2021

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,
1Eye Research Institute,Oakland University

Capitoli

  • 00:04Introduction
  • 00:36Tissue Preparation
  • 03:04Immunostaining and Refractive Index Matching
  • 04:05Mounting
  • 05:30Imaging
  • 06:36Results: Improved 3D Resolution of Fine Processes of Retina by Modified CLARITY Tissue Clearing Method
  • 08:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her præsenterer vi en protokol til at tilpasse klarhedsmetoden i hjernevævene til helmonterings nethinder for at forbedre kvaliteten af standard immunohistokemisk farvning og højopløsningsbilleddannelse af nethindeneuroner og deres subcellulære strukturer.

Tags

Keywords: ImmunostainingWhole-mount RetinaRetinal NeuronsMorphologyOptical Transparency3D ImagingCircuit WiringSubcellular StructuresEnucleationCorneaLensScleraRetina IsolationNitrocellulose FilterA4P0 SolutionSDSTriton X-100Primary AntibodyImmunostaining

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