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Etablierung eines epidermalen Sphäroidkultursystems mit hohem Durchsatz zur Modellierung der Plastizität von Keratinozyten-Stammzellen
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1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2Department of Dermatology,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine Baltimore, 4Department of Pathology,Albert Einstein College of Medicine, 5Department of Pediatrics,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina
Capitoli
- 00:04Introduction
- 00:27Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
- 02:37Generating Skin Epidermosphere Cultures in Vitro
- 05:02Epidermal Spheroid Re-Plating Assay
- 05:42Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
- 08:06Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
- 09:30Conclusion
Hier beschreiben wir ein Protokoll zur systematischen Kultivierung epidermaler Sphäroide in 3D-Suspensionskultur. Dieses Protokoll hat weitreichende Anwendungen für den Einsatz in einer Vielzahl von epithelialen Gewebetypen und für die Modellierung mehrerer menschlicher Krankheiten und Zustände.
