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从预成型微气泡生产膜过滤相移十氟丁烷纳米滴

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Bioingegneria

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JoVE Journal Bioingegneria

Production of Membrane-Filtered Phase-Shift Decafluorobutane Nanodroplets from Preformed Microbubbles

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从预成型微气泡生产膜过滤相移十氟丁烷纳米滴

DOI:

10.3791/62203-v

•

07:10 min

•

March 23, 2021

•

Darrah A. Merillat, Arvin Honari, Shashank R. Sirsi²

¹Department of Bioengineering,The University of Texas at Dallas, ²Department of Radiology,The University of Texas Southwestern

Capitoli

00:04Introduction
00:46Generating Microbubbles from Lipid Films
01:48Preparing and Priming the Extruder for Microbubble Condensation
03:13Pre-Cooling Microbubbles for Extrusion and Separating Droplets from Liposomes by Centrifugation
05:04Results: Phase-Shift Droplet Analysis of DFB and OFP Nanodroplets Using DLS, TRPS and Microscopy
06:19Conclusion

Summary

Traduzione automatica

该协议描述了一种使用探针尖端超声处理产生大量脂质封装的十氟丁烷微气泡的方法，然后使用高压挤出和机械过滤将它们冷凝成相移纳米滴。

Tags

Keywords: Membrane-filtered Phase-shift Decafluorobutane Nanodroplets Preformed Microbubbles Biomedical Applications Lipid Microbubbles Gas Microbubbles 200-nanometer Ceramic Filter High-pressure Extruder Nitrogen Gas Tank PBS Polydispersity Reduction Size Control Cost-effective Method

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