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Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices
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JoVE Journal Immunologia e infezioni
Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices

DOI:
10.3791/62207-v

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05:51 min

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April 12, 2021

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, , , , , , , , ,
1Department of Pathology, Immunology, and Laboratory Medicine,University of Florida, 2Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden, 3Institute of Physiology, Faculty of Medicine,Technische Universität Dresden, 4German Center for Diabetes Research (DZD), 5J. Crayton Pruitt Family Department of Biomedical Engineering,University of Florida

Capitoli

  • 00:05Introduction
  • 00:37Generation of the Mouse Pancreatic Slices
  • 02:18Immune Cell Recordings on Mouse Pancreatic Tissue Slices
  • 03:59Results: Viability and Immune Cell Activity of the Live Pancreatic Tissue Slices
  • 05:16Conclusion

Summary

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This study presents the application of live pancreatic tissue slices to the study of islet physiology and islet-immune cell interactions.

Tags

Keywords: Islet FunctionIslet-immune Cell InteractionsPancreatic IllnessesPancreatitisType I DiabetesType II DiabetesVibratomeExtracellular SolutionKrebs-Ringer Bicarbonate BufferTrypsin InhibitorSlice Culture MediumMicroscopeBrightfield ModeIslets

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