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Capitoli
- 00:04Introduction
- 01:04Collection of RPE
- 04:09Isolation of Primary Mouse RPE Cells
- 06:37Results: Morphological and Functional Validation of RPE Cells
- 07:38Conclusion
This protocol, which was originally reported by Fernandez-Godino et al. in 20161, describes a method to efficiently isolate and culture mouse RPE cells, which form a functional and polarized RPE monolayer within one week on Transwell plates. The procedure takes approximately 3 hours.
