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原发性小鼠视网膜色素上皮细胞的有效解剖与培养
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Biologia
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JoVE Journal Biologia
Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells
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原发性小鼠视网膜色素上皮细胞的有效解剖与培养

DOI:
10.3791/62228-v

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08:33 min

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February 10, 2021

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, ,
1Ocular Genomics Institute of Massachusetts Eye and Ear,Harvard Medical School

Capitoli

  • 00:04Introduction
  • 01:04Collection of RPE
  • 04:09Isolation of Primary Mouse RPE Cells
  • 06:37Results: Morphological and Functional Validation of RPE Cells
  • 07:38Conclusion

Summary

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该协议最初由费尔南德斯-戈迪诺等人于2016年1月报告，描述了一种有效分离和培养小鼠RPE细胞的方法，该细胞在一周内在Transwell板上形成功能和极化的RPE单层。手术大约需要3个小时。

Tags

Primary Mouse Retinal Pigment Epithelial CellsRPE Cell CultureEye DissectionAge-related Macular DegenerationHyaluronidaseHBSS BufferCornea IncisionOra Serrata

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