Rivista
/
Biologia
/
Partikelskabelonemulsificering muliggør mikrofluidisk-fri dråbe assays
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Partikelskabelonemulsificering muliggør mikrofluidisk-fri dråbe assays

DOI:
10.3791/62248-v

•

11:03 min

•

March 09, 2021

•

, ,
1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences,University of California, 2Chan Zuckerberg Biohub

Capitoli

  • 00:04Introduction
  • 00:44Preparation of Hydrogel Particles for Particle Templated Emulsification
  • 02:31Fabrication of the Custom Microfluidic Device
  • 04:37Fabrication of Templating Particles
  • 06:26Particle Templated Emulsification
  • 08:26Digital Droplet PCR and Analysis
  • 09:02Results: Sample Encapsulation Using Particle Templated Emulsification (PTE)
  • 10:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vand-i-olie dråbe assays er nyttige for analytisk kemi, enzym evolution, og enkelt celle analyse, men kræver typisk mikrofluidics at danne dråber. Her beskriver vi partikelskabelon emulsificering, en mikrofluidisk-fri tilgang til at udføre dråbe assays.

Tags

Particle Templated EmulsificationMicrofluidic-freeDroplet AssaysDigital Droplet PCREmulsificationPolyacrylamide ParticlesPhotoresistPDMSMicrofluidic FabricationPhotomaskPost Exposure BakingPGMEAIsopropanolPlasma Treatment

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62248?article_type=v

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
check_url/it/62248?article_type=v

Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
check_url/it/62248?article_type=v

Levedygtighed Analyser for celler i kultur
check_url/it/62248?article_type=v

In vitro Cell Migration og invasion Analyser
check_url/it/62248?article_type=v

Open source Single-partikel Analyse for Super-resolution Microscopy med VirusMapper
check_url/it/62248?article_type=v

Isolation og fluorescens Imaging for enkelt-partikel genopbygning af Chlamydomonas centrioler
check_url/it/62248?article_type=v

Droplet stregkodesystem-baserede enkelt celle Transcriptomics af voksne pattedyr væv
check_url/it/62248?article_type=v

Hurtig Lipid Droplet Isolation protokollen ved hjælp af en almindelig Organelle isolering Kit
check_url/it/62248?article_type=v

Dissektion og lipid dråbe farvning af Oenocytter i Drosophila larver
check_url/it/62248?article_type=v

Lr-ExM(Label-Retention Expansion Microscopy) muliggør billedbehandling i superopløsning og højeffektiv mærkning

Read Article