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通过交替粘性 - 惯性力喷射进行体外水凝胶微载体的3D打印

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Bioingegneria

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JoVE Journal Bioingegneria

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

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通过交替粘性 - 惯性力喷射进行体外水凝胶微载体的3D打印

DOI:

10.3791/62252-v

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05:32 min

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April 21, 2021

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Tiankun Liu*^1,2, Yongchun Shao*^1,2, Zitong Wang², Yuqiu Chen², Yuan Pang², Ding Weng², Wei Sun^2,3

¹Biomanufacturing Center, Dept. of Mechanical Engineering,Tsinghua University, ²Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing, ³Department of Mechanical Engineering,Drexel University

Capitoli

00:05Introduction
00:56Nozzles and Hydrogel Bioink Preparation
01:39Microdroplet Formation
02:20Microcarrier Formation
02:52Microcarrier Inoculation and Analysis
03:52Results: 3D-Printed In Vitro Hydrogel Microcarrier Analysis
04:48Conclusion

Summary

Traduzione automatica

这里介绍的是一种温和的3D打印技术，由交替的粘性惯性力驱动，以实现水凝胶微载体的构建。自制喷嘴具有灵活性，可轻松更换不同的材料和直径。可以获得直径为50-500μm的细胞结合微载体并收集以进行进一步培养。

Tags

Keywords: 3D Printing Hydrogel Microcarriers Alternating Viscous-inertial Force Jetting Bioprinting In Vitro Cell Expansion Microcarrier Preparation Sodium Alginate Crosslinking Cell Encapsulation A549 Cells Cell Tracker Dye

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