Rivista
/
Biologia
/
单一肌纤维培养测定法用于评估体外成人肌肉干细胞功能
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
Please note that all translations are automatically generated. Click here for the English version.

单一肌纤维培养测定法用于评估体外成人肌肉干细胞功能

DOI:
10.3791/62257-v

•

09:19 min

•

February 15, 2021

•

, , , , ,
1Leibniz Institute on Aging, Fritz-Lipmann-Institute

Capitoli

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

在该协议中，描述了肌肉干细胞的体外培养和功能分析方法，该方法保留了它们与其内源性生态位的大部分相互作用。

Tags

Keywords: Muscle Stem CellsSingle Myofiber CultureEx VivoNicheSiRNA TransfectionPasteur PipettesHorse SerumEthanolTibialis AnteriorEDL MuscleDigestionIsolation MediumMyofibers

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62257?article_type=v

文化早期鸡胚体外（新文化）的方法
check_url/it/62257?article_type=v

成人和胚胎骨骼的肌肉Microexplant文化和骨骼肌肉干细胞的分离
check_url/it/62257?article_type=v

慢病毒介导的击倒在体外红细胞
check_url/it/62257?article_type=v

在体内电穿孔Morpholinos到成年斑马鱼视网膜
check_url/it/62257?article_type=v

体外评估对离体骨骼肌的收缩，疲劳性和交替“
check_url/it/62257?article_type=v

单个肌纤维和成人骨骼肌卫星细胞的分离和培养
check_url/it/62257?article_type=v

一种新型的<em>体外</em小鼠胚胎心脏的培养方法
check_url/it/62257?article_type=v

在鼠标提睾肌为外周干细胞迁移分析的微循环活体显微镜
check_url/it/62257?article_type=v

分离，培养和移植肌卫星细胞
check_url/it/62257?article_type=v

从人骨骼肌隔离血管源性多能前体

Read Article