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Enkelt Myofiber Kultur Assay til vurdering af voksne muskelstamcellefunktionalitet Ex Vivo
JoVE Journal
Biologia
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JoVE Journal Biologia
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Enkelt Myofiber Kultur Assay til vurdering af voksne muskelstamcellefunktionalitet Ex Vivo

DOI:
10.3791/62257-v

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09:19 min

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February 15, 2021

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, , , , ,
1Leibniz Institute on Aging, Fritz-Lipmann-Institute

Capitoli

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Summary

Traduzione automatica

Traduzione automatica

I denne protokol beskrives en in vitro-dyrkningsmetode og funktionel analysemetode for muskelstamceller, som bevarer de fleste af deres interaktioner med deres endogene niche.

Tags

Muscle Stem CellsSingle Myofiber CultureEx VivoNicheSiRNA TransfectionPasteur PipettesHorse SerumEthanolTibialis AnteriorEDL MuscleDigestionIsolation MediumMyofibers

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