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Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging
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Neuroscienze
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JoVE Journal Neuroscienze
Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging

Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging

DOI:
10.3791/62267-v

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11:57 min

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February 24, 2021

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, , , , , , , , , , ,
1Physics for Medicine Paris, ESPCI Paris, INSERM, CNRS,PSL Research University, 2Institute of Psychiatry and Neurosciences of Paris, INSERM U1266,Université de Paris, 3Iconeus, 4Department of Pharmacology and Toxicology,Jena University Hospital – Friedrich Schiller University Jena

Capitoli

  • 00:05Introduction
  • 01:22Animal Preparation
  • 03:58Probe Positioning
  • 04:34Angiographic Scan and Atlas Registration
  • 05:49Brain Positioning System (BPS)
  • 07:14Task-Evoked Experiment: Whisker Stimulation
  • 08:144D Functional Connectivity
  • 09:08Results: Transcranial Activation Maps and rCBV Time Course Following Whiskers Stimulation in an Anesthetized and Awake Behaving Mouse
  • 11:08Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the quantification of volumetric cerebral hemodynamic variations in the mouse brain using functional ultrasound (fUS). Procedures for 3D functional activation map following sensory stimulation as well as resting-state functional connectivity are provided as illustrative examples, in anesthetized and awake mice.

Tags

Keywords: Functional UltrasoundTranscranialWhole-brain3D ActivationFunctional ConnectivityMiceAnesthetizedAwakeNeuroimagingCerebral Blood VolumePlane Wave ImagingFMRIPortabilityHead FixationStereotactic FrameDental CementHead PlateAnesthesia

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