Rivista
/
Neuroscienze
/
A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
JoVE Journal
Neuroscienze
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

DOI:
10.3791/62268-v

•

06:54 min

•

June 03, 2021

•

,
1Department of Anatomy, School of Biomedical Sciences,University of Otago

Capitoli

  • 00:00Introduction
  • 01:49Preparation of Buffers and Reagents
  • 02:02Preparation of Samples
  • 02:56KCl-mediated Depolarization of Isolated Synaptic Terminals
  • 03:21Fixation and Phalloidin Staining of Samples
  • 04:40Fluorometric Analysis and Light Scattering
  • 05:20Data Analysis
  • 06:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

We report a simple, time-efficient and high-throughput fluorescence spectroscopy-based assay for the quantification of actin filaments in ex vivo biological samples from brain tissues of rodents and human subjects.

Tags

Keywords: ActinCytoskeletonF-actinG-actinPolymerizationFluorescence SpectroscopyPhalloidinSynaptic TerminalsSynaptosomesSynaptoneurosomesNeuropathologyNeuronal Physiology

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62268?article_type=v

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
check_url/it/62268?article_type=v

Double Fluorescence in situ Hybridization in Fresh Brain Sections
check_url/it/62268?article_type=v

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
check_url/it/62268?article_type=v

A General Method for Evaluating Incubation of Sucrose Craving in Rats
check_url/it/62268?article_type=v

In vivo Optogenetic Stimulation of the Rodent Central Nervous System
check_url/it/62268?article_type=v

Personalized Needles for Microinjections in the Rodent Brain
check_url/it/62268?article_type=v

High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay
check_url/it/62268?article_type=v

A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay
check_url/it/62268?article_type=v

Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries
check_url/it/62268?article_type=v

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Read Article