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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
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Capitoli
- 00:00Introduction
- 01:11Protocol
- 03:52Risultati
- 05:38Conclusion
The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.
