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Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
JoVE Journal
Biologia
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JoVE Journal Biologia
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

DOI:
10.3791/62277-v

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05:56 min

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February 27, 2021

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, , ,
1Department of Plant Pathology and Microbiology,Iowa State University

Capitoli

  • 00:04Introduction
  • 00:39Preparation of Seedling and Agrobacterium
  • 02:06Injection of Agrobacterium and Infection Detection
  • 03:36Results: Detection of Infection Lesion and Gene Suppression Analysis
  • 05:22Conclusion

Summary

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An Agrobacterium-based injection (agroinjection) protocol is presented for the inoculation of foxtail mosaic virus and sugarcane mosaic virusclones into maize seedlings. Inoculation in this manner leads to viral infection, virus-induced gene silencing of marker genes, and viral overexpression of GFP.

Tags

Keywords: MaizeAgroinoculationFoxtail Mosaic VirusSugarcane Mosaic VirusViral VectorsRecombinantInoculationColeoptileGene ExpressionRT-PCRGFP

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