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1Department of Physiology & The Institute for Digital Medicine (WisDM),National University of Singapore, 2College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute,National University of Singapore, 4Institute of Bioengineering and Nanotechnology,A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP,Singapore-MIT Alliance for Research and Technology
Capitoli
- 00:04Introduction
- 00:45Portal Vein Cannulation
- 03:54Liver Perfusion, Digestion, Hepatocyte Isolation
- 05:30Results: Estimation of Purity and Function of Isolated Hepatocytes
- 07:07Conclusion
Denne protokollen beskriver bruken av et spesielt intravenøst kateter, standardisert steril engangsrør, temperaturkontroll supplert med sanntidsovervåking og et alarmsystem for to-trinns kollagennaseperfusjonsprosedyre for å forbedre konsistensen i levedyktigheten, utbyttet og funksjonaliteten til isolerte primærrottehedopatocytter.
