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急性小鼠胰腺组织切片钙动力学的共聚焦激光扫描显微镜
JoVE Journal
Biologia
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JoVE Journal Biologia
Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
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急性小鼠胰腺组织切片钙动力学的共聚焦激光扫描显微镜

DOI:
10.3791/62293-v

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10:49 min

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April 13, 2021

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, , , , ,
1Institute of Physiology, Faculty of Medicine,University of Maribor, 2Faculty of Natural Sciences and Mathematics,University of Maribor, 3Center for Physiology and Pharmacology,Medical University of Vienna

Capitoli

  • 00:05Introduction
  • 01:04Injection of Pancreas with Agarose
  • 02:57Tissue Slice Preparation
  • 05:13Calcium Dye Preparation and Loading
  • 06:22Calcium Imaging
  • 08:17Results: Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
  • 10:03Conclusion

Summary

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我们介绍了急性胰腺组织切片的制备及其在共聚焦激光扫描显微镜中的应用，以研究大量活细胞中钙动力学，时间长，时空分辨率高。

Tags

Confocal Laser Scanning MicroscopyCalcium DynamicsLive Cell Calcium ImagingIntercellular WavesMulticellular Functional ConnectivityEnzymatic And Mechanical Stress ReductionDiabetesBrainPituitaryAgarose InjectionCommon Bile DuctDuodenal PapillaExtracellular SolutionCarbogenEuthanized Adult MouseLaparotomyAgarose Hardening

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