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VMXm 光束线微晶样品制备管道
JoVE Journal
Biochimica
This content is Free Access.
JoVE Journal Biochimica
A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
DOI:

09:00 min

June 17, 2021

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Capitoli

  • 00:05Introduction
  • 00:42Instrument and Blotting Parameter Set-up
  • 02:53Crystal Harvest Process
  • 04:01Light Microscopy for Sample Density Assessment
  • 04:30Scanning Electron Microscopy for Sample Assessment
  • 05:38Grid Preparation for the Diffraction Experiments at VMXm
  • 06:47Results: Analysis of the Microcrystals on the Grids
  • 08:24Conclusion

Summary

Traduzione automatica

数据的信号与噪声比是执行微晶 X 射线衍射测量中最重要的考虑因素之一。VMXm 光束线为此类实验提供了低噪音环境和微束。在这里,我们描述了 VMXm 和其他微焦大分子晶体光束线的安装和冷却微晶体的样品制备方法。

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