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Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues
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JoVE Journal Bioingegneria
Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

DOI:
10.3791/62307-v

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09:30 min

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February 18, 2021

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, , ,
1Institute of Biomedical Engineering,University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, 3Department of Cell and Systems Biology,University of Toronto

Capitoli

  • 00:04Introduction
  • 00:42Preparation of Myotactic Culture Wells and Reagents for Human Skeletal Muscle Microtissues (hMMTs) Seeding
  • 02:51Preparation of Cells for hMMTs Seeding
  • 05:24Seeding hMMTs
  • 07:24Results: Measurement of Contractile Force and Calcium Handling Within the Myotactic Platform
  • 08:57Conclusion

Summary

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This manuscript describes a detailed protocol to produce arrays of 3D human skeletal muscle microtissues and minimally invasive downstream in situ assays of function, including contractile force and calcium handling analyses.

Tags

Muscle BiologyDisease ModelingCandidate Molecule TestingContractile ForceCalcium TransientsLongitudinal StudiesBasement Membrane ExtractThrombinFibrinogen

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