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使用稀疏腺相关病毒标记遗传靶向视网膜细胞群的神经元树化延时成像
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Time-Lapse Imaging of Neuronal Arborization using Sparse Adeno-Associated Virus Labeling of Genetically Targeted Retinal Cell Populations
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使用稀疏腺相关病毒标记遗传靶向视网膜细胞群的神经元树化延时成像

DOI:
10.3791/62308-v

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13:13 min

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March 19, 2021

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,
1Program for Neuroscience and Mental Health,Hospital for Sick Children, 2Department of Molecular Genetics,University of Toronto

Capitoli

  • 00:04Introduction
  • 00:58Adeno-Associated Virus (AAV) Preparation and Intravitreal Injection of Neonatal Mice
  • 03:13Retinal Dissections for Live-Imaging Experiment
  • 05:04Retinal Flat-Mount Preparation
  • 06:35Time-Lapse Confocal Imaging of Live Whole-Mount Retina Preparations
  • 08:33Image Deconvolution and Post-Processing in ImageJ
  • 11:16Results: Representative Results After Neonatal Intraocular Injection, Time-Lapse Imaging, and Deconvolution
  • 12:13Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提出了一种通过延时共聚焦显微镜研究产后小鼠视网膜外植体中神经突形态发生的方法。我们描述了一种使用重组腺相关病毒载体（以 Cre依赖性方式表达膜靶向荧光蛋白）对视网膜细胞类型及其精细过程进行稀疏标记和获取的方法。

Tags

Time-lapse ImagingNeuronal ArborizationSparse Adeno-associated VirusGenetically Targeted Retinal Cell PopulationsSingle-cell LabelingDendritic ArborAxonal ArborNeuronal MorphogenesisConfocal Live ImagingNeonatal Arbor MorphologiesCentral Nervous SystemCre Mouse LineRecombinase-dependent AAVFluorescent ProteinsPlasma MembraneAAV DilutionFast Green FCF DyeNeonatal MiceCorneaIntravitreal SpaceRetinal ACSFCarbogen

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