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Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
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Capitoli
- 00:00Introduction
- 00:50Preparation of the Bacteria Strain
- 02:21Isolation of Neutrophils
- 07:41Measurement of ROS
- 09:51Representative Results
- 12:22Conclusion
Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis. During the bacterial infection, reactive oxygen species produced by neutrophils play a major bactericidal role. Here we introduce a method to detect the reactive oxygen species in neutrophils in response to meningitis E. coli.
