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Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

DOI:
10.3791/62332-v

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07:07 min

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March 12, 2021

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, ,
1Masonic Medical Research Institute

Capitoli

  • 00:04Introduction
  • 01:09Brown Adipocytes (BAs) Isolation Procedure
  • 02:14RNA and Protein Isolation from BAs
  • 04:28Results: Validation of BA Isolation Method with Microscopy and Gene Expression Analysis
  • 06:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

This study describes a new method of isolating murine brown adipocytes for gene and protein expression analysis.

Tags

Keywords: Brown AdipocytesGene ExpressionProtein ExpressionAdipocyte IsolationIodixanol GradientTRIzolRNA IsolationProtein Isolation

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