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DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
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JoVE Journal Bioingegneria
DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

DOI:
10.3791/62348-v

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06:53 min

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March 20, 2021

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, , , , ,
1Department of Chemistry,Emory University, 2Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University

Capitoli

  • 00:04Introduction
  • 00:41Surface Preparation and Activation of the Coverslip
  • 04:37Imaging Cell Receptor Forces
  • 05:21Results: Estimation of Quality and Functionalization of the DNA Probe Surface
  • 06:17Conclusion

Summary

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This paper describes a detailed protocol for using DNA-based tension probes to image the receptor forces applied by immune cells. This approach can map receptor forces >4.7pN in real-time and can integrate forces over time.

Tags

Keywords: DNA Tension ProbesMechanotransductionMechanobiologyFluorescence MicroscopyForce TransmissionImmortalized CellsPrimary CellsPolytetrafluoroethylenePiranha SolutionAminopropyltriethoxysilaneAmineLipoic Acid PEG NHSMPEG NHS

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