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金刚石光源下光束线I23真空长波长晶体学的样品制备和转移实验方案

JoVE Journal
Biochimica

This content is Free Access.

JoVE Journal Biochimica

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

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金刚石光源下光束线I23真空长波长晶体学的样品制备和转移实验方案

DOI:

10.3791/62364-v

•

10:32 min

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April 23, 2021

•

Ramona Duman, Christian M. Orr, Vitaliy Mykhaylyk, Kamel El Omari, Robert Pocock, Vinay Grama, Armin Wagner

¹Diamond Light Source Ltd, Harwell Science & Innovation Campus

Capitoli

00:04Introduction
01:15Crystal Harvesting
01:58Loading of SH from Combipuck to the Transfer Block
03:44Loading of Transfer Blocks into the Vacuum Vessel
08:08Results: Diffraction Images and Structure Solution of Thaumatin
09:41Conclusion

Summary

Traduzione automatica

在这里，我们提出了一种用于低温样品制备和将晶体转移到金刚石光源光束线I23上的真空终端站的方案，用于长波长高分子X射线晶体学实验。

Tags

Sample Preparation Sample Transfer Macromolecular Crystallography Long-wavelength Beamline I23 Diamond Light Source Cryogenic Temperature Vacuum Environment CombiPuck Magnetic Wand Liquid Nitrogen Dry Shipper Sample Holder Adapter Puck Separator Tool

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