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优化低温电子断层扫描数据采集策略
JoVE Journal
Biologia
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JoVE Journal Biologia
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
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优化低温电子断层扫描数据采集策略

DOI:
10.3791/62383-v

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08:16 min

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March 19, 2021

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, , ,
1Structural and Computational Biology Unit,European Molecular Biology Laboratory, 2Electron Microscopy Core Facility,European Molecular Biology Laboratory, 3Imaging Centre,European Molecular Biology Laboratory

Capitoli

  • 00:05Introduction
  • 00:38Selecting the Targets
  • 01:31Generate Virtual Maps
  • 02:07Microscope Tuning
  • 02:43Set up Navigator
  • 04:05Set the Focus/Track Positions
  • 05:00Set up Additional Scripts
  • 05:27Run
  • 06:19Results: Optimization of Cryogenic Electron Tomography Data Acquisition
  • 07:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

低温电子断层扫描对大规模数据收集的需求不断增加，这需要高通量图像采集程序。这里描述的是一个协议，旨在实现高级获取策略的最新发展，旨在最大限度地提高断层数据收集的时间效率和吞吐量。

Tags

Keywords: Cryogenic Electron TomographyData AcquisitionSerialEMVirtual Anchor MapsComa-free AlignmentAstigmatism CorrectionTilt Series AcquisitionPreview MapsFocus/track Distance

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