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Journal / Biochemistry / Ganglioside Extraction, Purification and Profiling
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Biochemistry

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JoVE Journal Biochemistry
Ganglioside Extraction, Purification and Profiling
 

Ganglioside Extraction, Purification and Profiling

Article DOI: 10.3791/62385-v • 10:05 min • March 12th, 2021
March 12th, 2021 •
*1, *1, 1,2
1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 2Department of Neuroscience, Johns Hopkins University School of Medicine
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Gangliosides are sialic acid-bearing glycosphingolipids that are particularly abundant in the brain. Their amphipathic nature requires organic/aqueous extraction and purification techniques to ensure optimal recovery and accurate analyses. This article provides overviews of analytic and preparative scale ganglioside extraction, purification, and thin layer chromatography analysis.

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Keywords: Ganglioside Extraction Ganglioside Purification Ganglioside Profiling Cell Surface Lipids Nerve Cell Lipids Cell Signaling Cell-cell Recognition Polar Lipid Analysis Reverse-phase Cartridge Chromatography Solvent Extraction Partitioning Solid-phase Extraction
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