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Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
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JoVE Journal Immunologia e infezioni
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

DOI:
10.3791/62387-v

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10:37 min

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October 20, 2021

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, , ,
1Department of Infectious Diseases,Southwest Hospital, Third Military Medical University (Army Medial University), 2Chongqing Key Laboratory for Research of Infectious Diseases

Capitoli

  • 00:04Introduction
  • 00:51Isolation of PBMCs and In Vitro Expansion Using an HBV Peptide Matrix
  • 03:55Analysis of HBV-specific CD4 T-Cell Responses by Intracellular Flow Cytometry
  • 05:54Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
  • 08:07Results: Identification of HBV-specific HLA-DR Restricted CD4 T-Cell Epitopes
  • 10:06Conclusion

Summary

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Based on a hepatitis B virus (HBV)-derived peptide matrix, HBV-specific CD4 T-cell responses could be evaluated in parallel with identification of HBV-specific CD4 T-cell epitopes.

Tags

HBV-specific CD4 T-cell ResponsesHLA-DR-restricted CD4 T-cell EpitopesChronic HBV InfectionPBMC IsolationPeptide MatrixCD4 T-cell Response AnalysisHBV-derived Peptide PoolsIL-2IL-796-well Plates

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