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Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells

DOI:
10.3791/62391-v

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04:23 min

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March 31, 2021

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, , ,
1Department of Cell Biology,University of Virginia, 2Cardiovascular Research Center,University of Virginia, 3Department of Medicine,Yale University School of Medicine, 4Department of Genetics,Yale University School of Medicine, 5Yale Cardiovascular Research Center,Yale University School of Medicine

Capitoli

  • 00:04Introduction
  • 00:25Differentiation of Human Embryonic Stem Cells to Hemogenic Endothelial Cells
  • 01:17FACS-Isolation of Hemogenic Endothelial Cells
  • 02:56Results: Hematopoietic Potential of Hemogenic Endothelial Cells
  • 03:54Conclusion

Summary

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Presented here is a simple protocol for the directed differentiation of hemogenic endothelial cells from human pluripotent stem cells in approximately 1 week.

Tags

Hemogenic Endothelial CellsHuman Pluripotent Stem CellsDirected DifferentiationFACSCD45CD31VE-cadherinC-KitCD34KDRMethylcelluloseColony Forming UnitBlast Forming UnitHematopoietic Progenitor Cells

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