Rivista
/
Biologia
/
Generation of Human Kidney Tubuloids from Tissue and Urine
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Generation of Human Kidney Tubuloids from Tissue and Urine

Generation of Human Kidney Tubuloids from Tissue and Urine

DOI:
10.3791/62404-v

•

08:34 min

•

April 16, 2021

•

,
1Princess Máxima Center for Pediatric Oncology, 2Oncode Institute

Capitoli

  • 00:04Introduction
  • 00:50Generation of Human Kidney Tubuloids From Tissue
  • 03:45Generation of Human Kidney Tubuloids From Urine
  • 05:02Expansion of Tubuloid Cultures
  • 07:01Results: Successful Generation of Human Kidney Tubuloids From Tissue and Urine
  • 07:55Conclusion

Summary

Traduzione automatica

Traduzione automatica

Human kidney tubuloid cultures represent a valuable in vitro model to study kidney physiology and disease. Tubuloids can be established from kidney tissue (healthy and diseased) as well as urine, the latter representing an easily obtainable and less invasive source of research material.

Tags

Kidney TubuloidsUrineKidney Disease ModelingKidney PhysiologyBasement Membrane ExtractCollagenaseCell Culture

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62404?article_type=v

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
check_url/it/62404?article_type=v

Generation of Single-Cell Suspensions from Mouse Neural Tissue
check_url/it/62404?article_type=v

Generation of Human CD40-activated B cells
check_url/it/62404?article_type=v

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
check_url/it/62404?article_type=v

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells
check_url/it/62404?article_type=v

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
check_url/it/62404?article_type=v

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
check_url/it/62404?article_type=v

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
check_url/it/62404?article_type=v

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues
check_url/it/62404?article_type=v

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Read Article