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Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
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Biologia
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JoVE Journal Biologia
Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

DOI:
10.3791/62410-v

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10:32 min

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April 02, 2021

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,
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center,Sun Yat-Sen University

Capitoli

  • 00:05Introduction
  • 00:58Preparation of Retinal Whole Mounts and Immunofluorescence Staining
  • 05:36In Vivo Imaging with Fluorescein Fundus Angiography (FFA)
  • 07:02FAA Image Processing
  • 08:40Results: Vaso-Obliteration (VO) and Neovascularization (NV) in the Oxygen-Induced Retinopathy (OIR) Mouse Model
  • 10:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes a detailed method for the preparation and immunofluorescence staining of mice retinal flat mounts and analysis. The use of fluorescein fundus angiography (FFA) for mice pups and image processing are described in detail as well.

Tags

Keywords: Retinal VesselsOxygen-induced RetinopathyMouse ModelRetinal Whole MountingRetinal VasculatureRetinal SeparationEye Dissection

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