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Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
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Biologia
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JoVE Journal Biologia
Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

DOI:
10.3791/62418-v

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08:11 min

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August 07, 2021

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, ,
1Structural Genomics Consortium,University of Toronto, 2Department of Pharmacology and Toxicology,University of Toronto

Capitoli

  • 00:00Introduction
  • 00:47Cell Lysate Preparation
  • 03:06Western Blot Analysis
  • 06:41Representative Results
  • 07:36Conclusions

Summary

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These protocols provide the methodology used to assess the enzymatic activity of individual members of the protein arginine methyltransferase (PRMT) family in cells. Detailed guidelines on assessing PRMT activity using endogenous and exogenous biomarkers, methyl-arginine recognizing antibodies, and inhibitor tool compounds are described.

Tags

Protein Arginine MethyltransferaseEnzymatic ActivityQuantitative Fluorescent Western BlottingBiomarkersCell LysisProtein ConcentrationGel ElectrophoresisWestern Blot Transfer

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