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Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro
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Biologia
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JoVE Journal Biologia
Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

DOI:
10.3791/62419-v

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05:47 min

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May 10, 2021

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,
1Cardiovascular Research Center,The Icahn School of Medicine at Mount Sinai, 2Diabetes, Obesity and Metabolism Institute, Department of Medicine,The Icahn School of Medicine at Mount Sinai, 3Graduate School of Biomedical Sciences,The Icahn School of Medicine at Mount Sinai

Capitoli

  • 00:04Introduction
  • 00:43Isolation of Valve Cells
  • 03:35In vitro Calcification Assay
  • 04:23Results: Identification of Mouse Valve Cell Phenotype and Calcification Assay
  • 05:02Conclusion

Summary

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This article describes the isolation of mouse aortic valve cells by a two-step collagenase procedure. Isolated mouse valve cells are important for performing different assays, such as this in vitro calcification assay, and for investigating the molecular pathways leading to aortic valve mineralization.

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Mouse Interstitial Valve CellsAortic Valve CalcificationIn VitroTwo-step DigestionAortic Valve IsolationCollagenaseHEPESCell CultureCell SeparationCentrifugation

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