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Immunology and Infection

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JoVE Journal Immunology and Infection
Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model
 

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

Article DOI: 10.3791/62420-v • 07:39 min • April 6th, 2021
April 6th, 2021 •
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1Department of Molecular Medicine, Morsani College of Medicine, University of South Florida

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Synthetic cystic fibrosis sputum medium (SCFM2) can be utilized in combination with both confocal laser scanning microscopy and fluorescence-activated cell sorting to observe bacterial aggregates at high resolution. This paper details methods to assess aggregate populations during antimicrobial treatment as a platform for future studies.

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Keywords: Pseudomonas Aeruginosa Infection Model Real-time Assessment Antibiotic Resistance Mucin Synthetic Cystic Fibrosis Sputum Medium Confocal Laser Scanning Microscope Bacterial Aggregates Optical Density Log Phase PBS Wash Static Incubation
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