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Hurtig frysning ved hjælp af Sandwich Frysning Device for god ultrastrukturel bevarelse af biologiske prøver i ElektronMikroskopi
JoVE Journal
Biologia
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JoVE Journal Biologia
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Hurtig frysning ved hjælp af Sandwich Frysning Device for god ultrastrukturel bevarelse af biologiske prøver i ElektronMikroskopi

DOI:
10.3791/62431-v

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09:03 min

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July 19, 2021

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, , , , ,
1Medical Mycology Research Center,Chiba University, 2Marine Works Japan Ltd.

Capitoli

  • 00:04Introduction
  • 00:48Rapid Freezing of Cell Suspensions for Freeze-Substitution
  • 07:19Results: Ultrastructural Preservation of Biological Specimens in Electron Microscopy Using Sandwich Freezing Device
  • 08:03Conclusion

Summary

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Her viser vi, hvordan man bruger sandwichfrysningsanordningen til hurtig frysning af biologiske prøver, herunder bakterier, gær, dyrkede celler, isolerede celler, dyre- og humane væv og vira. Vi viser også, hvordan man forbereder prøver til ultratynde afsnit efter hurtig frysning.

Tags

Keywords: Rapid FreezingSandwich Freezing DeviceUltrastructural PreservationBiological SpecimensElectron MicroscopyCell FixationIce Crystal FormationCopper DiscsGlow DischargeCentrifugationPropane FreezingGlutaraldehyde FixationFreeze-substitution

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