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使用荧光染料，罗达明B，研究雄性 伊蚊 的交配竞争力
JoVE Journal
Biologia
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JoVE Journal Biologia
Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes
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使用荧光染料，罗达明B，研究雄性 伊蚊 的交配竞争力

DOI:
10.3791/62432-v

•

07:10 min

•

May 07, 2021

•

, , ,
1Environmental Health Institute,National Environmental Agency

Capitoli

  • 00:04Introduction
  • 00:41Preparation of Male and Female Mosquitoes
  • 01:11Preparation of 0.2% RhB Sucrose Solution and Feeding of Male Mosquitoes
  • 01:52Checking for RhB Fluorescence in Male Mosquitoes
  • 02:46Mating Competitiveness Assay
  • 05:36Results: Assessment of Mating Competitiveness in Male Mosquitoes
  • 06:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提出了一个协议，研究雄性 伊蚊 的交配竞争力使用荧光染料作为标记。雌性蚊子接触有标记和无标记的雄性进行交配。交配后，在荧光显微镜下检查他们的精子，以确定他们的交配伙伴。

Tags

Rhodamine BAedes AegyptiMating CompetitivenessMosquito Population SuppressionQuality ControlStrain EvaluationMale MosquitoesFemale MosquitoesSucrose SolutionFluorescence MicroscopyMating Assay

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