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分离人原代瓣膜细胞进行体外疾病建模
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation of Human Primary Valve Cells for In vitro Disease Modeling
DOI:

07:31 min

April 16, 2021

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Capitoli

  • 00:04Introduction
  • 00:34Tissue Preparation and Processing
  • 01:32Valve Endothelial Cell (VEC) Isolation, Expansion, and Confirmation
  • 04:01Valve Interstitial Cell (VIC) Isolation, Expansion, and Confirmation
  • 05:40Results: Analysis of Isolated Human Primary Valve Cells
  • 06:47Conclusion

Summary

Traduzione automatica

该协议描述了在外科主动脉瓣置换手术期间或从尸体组织中提取的人主动脉瓣的收集,以及随后对患者特异性原发性瓣膜内皮和间质细胞的分离、扩增和表征。其中包括有关确保细胞活力和表型特异性所需过程的重要细节。

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