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1Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute,University of Pittsburgh, 2Division of Cardiac Surgery, Department of Cardiothoracic Surgery,University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, 3Department of Bioengineering,University of Pittsburgh
Capitoli
- 00:04Introduction
- 00:34Tissue Preparation and Processing
- 01:32Valve Endothelial Cell (VEC) Isolation, Expansion, and Confirmation
- 04:01Valve Interstitial Cell (VIC) Isolation, Expansion, and Confirmation
- 05:40Results: Analysis of Isolated Human Primary Valve Cells
- 06:47Conclusion
Denne protokol beskriver samlingen af humane aortaklapper ekstraheret under kirurgiske aortaklapudskiftningsprocedurer eller fra kadavervæv og den efterfølgende isolering, ekspansion og karakterisering af patientspecifikke primære ventilendotel- og interstitielle celler. Inkluderet er vigtige detaljer om de processer, der er nødvendige for at sikre cellelevedygtighed og fænotypespecificitet.
