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Tumortransplantatie voor het beoordelen van de dynamiek van tumorinfiltrerende CD8 + T-cellen bij muizen
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1Institute of Immunology,Third Military Medical University, 2Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital,Sun Yat-Sen University, 3Shigatse Branch, Xinqiao Hospital,Third Military Medical University, 4Department of Emergency Medicine, Southwest Hospital,Third Military Medical University, 5Cancer Center,The General Hospital of Western Theater Command
Capitoli
- 00:04Introduction
- 00:56Ectopic Tumor Implantation of B16F10-OVA Cells in the Inguinal Region of Mice
- 02:23Adoptive Transfer of Congenically Marked OT-I T Cells into Tumor-Bearing Mice
- 03:26Dissect Tumor Mass from Tumor-Bearing Donor Mice
- 04:31Subcutaneous Transplantation of Donor-Derived Tumor onto the Tumor-Matched Recipient Mice
- 06:14Results: For Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
- 07:04Conclusion
Hier presenteren we een tumortransplantatieprotocol voor de karakterisering van tumor-inherente en periferie-afgeleide tumor-geïnfiltreerde lymfocyten in een muistumormodel. Specifieke tracering van de instroom van van de ontvanger afgeleide immuuncellen met flowcytometrie onthult de dynamiek van de fenotypische en functionele veranderingen van deze cellen tijdens antitumorimmuunresponsen.
